Dating cup size
In recent years, several AMS labs have worked on modifications to the graphitisation and AMS measurement process for smaller samples containing .
This involves demineralisation of either powdered bone or bone chunks using hydrochloric acid (HCl) followed by gelatinisation of the collagen in weakly acidic water and freeze-drying of the final extract.
Analysis with FTIR was performed for all collagen extracts; each extract dated had a spectra characteristic of well-preserved collagen when compared to library spectra (see Supplementary Fig. Considering the collagen yields and For each of the bones, several collagen extracts (bone weight ranging from 37–100 mg, marked with asterisks in Fig. Between two and four replicates were measured containing ca.
During the gelatinisation stage (step 3), the collagen yield was higher from aliquots which were removed from the heater block as soon as solubilisation had occurred compared to those left on the heater block for 20 h as per our standard protocol for C dating (Supplementary Dataset S1).Small aliquots (N) were used to evaluate the extracts from the different methods.In addition, Fourier Transform Infrared Spectroscopy (FTIR) was used to double check the preservation of the extracted collagen, and to detect the presence of possible carbon contaminants. 1d: R-EVA 548) the pretreatment was softened in order to minimise collagen loss during the extraction.The gas ages obtained were compared to one or more graphite dates measured from collagen extracted from 500–700 mg bone material (Supplementary Dataset S2).Discussed here are measurements made from collagen extracted from solid pieces of bone.